homology inherited Protein-Protein Interaction
The hiPPI method takes advantage of the Gene3D families of structurally conserved proteins as well as multiple sources of protein-protein physical interaction (PPI) data to reliably infer (‘inherit’) novel protein-protein interactions from homologues. hiPPI exploits the Gene3D protein families (G3D_families) datasets. These are families of proteins with similar multi-domain architectures generated using an automated, but conservative, clustering procedure. Interactions are only inherited between proteins belonging to the same Gene3D family, even though there may be recognisable sequence similarity with a protein in another cluster. This step helps to reduce the amount of noise produced by attempting to inherit from overly-distantly related sequences.
The interaction dataset is formed from a merger of the PPI resources from MIPS, IntAct, HPRD and MINT protein-protein interaction datasets, obtained from the Gene3D database.
Click here for a technical report (PDF, 1MB) which describes the hiPPI algorithm in detail.
